The effects of fermented feedstuff derived from Citri Sarcodactylis Fructus by-products on growth performance, intestinal digestive enzyme activity, nutrient utilization, meat quality, gut microbiota, and metabolites of broiler chicken

This research aimed to assess the impact of fermented Citri Sarcodactylis Fructus by-products (FCSF) on growth performance, gut digestive enzyme activity, nutrient utilization efficiency, microbiota, and their metabolites in broiler chickens. A total 1,080 male chickens were allocated into four groups (T1–T4) consisting 6 replicates per group, each containing 45 The basal diet was provided group T1, while T2, T3, T4 supplemented with 1%, 3%, 5% FCSF diet, respectively. experimental period 42 days. findings revealed that supplementing improved FW ADG chickens, led a reduction F/G, ADFI, mortality rate ( p < 0.05). Furthermore, supplementation 3% thigh yield, semi-eviscerated carcass slaughter lipase activity duodenum ileum birds Additionally, enhanced protease broilers Moreover, phosphorus, dry matter, crude protein, ash feed by Compared control reduced serine content chicken breast meat Supplementing 1% significantly increased C14:0, C14:1, C20:1 compared other levels C20:4n6 C23:0 lower than T1 ∑ω-3PUFA decreased after 16SrDNA showed ACE, Chao1, Shannon indices cecum also abundance phylum Desulfobacterota genera Coprobacter Prevotella Metabolomic analysis microbiota upregulated downregulated 16 12 metabolic pathways 3 In summary our indicate can improve performance enhancing intestinal utilization, improving microbial diversity, influencing metabolism microbiota.